Study on Rapid Activating Molecular Sieve Chromatography Column and its Application 分子筛色谱柱快速活化方法的研究及应用
Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography. 方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。
Determination of Molecular Sieve Diffusivity by Gas Chromatography 气相色谱法测定分子筛扩散系数
This method uses molecular sieve chromatography to desalt and cleans, transforms the complicated procedure of using dialysis in desalting and ion& exchange chromatography in cleansing cytochrome C and has the advantages of simplicity, quickness and high level purity. 本法改变了透析法脱盐和离子交换柱层析等传统的分离纯化细胞色素C的繁琐过程,采用了分子筛色谱法脱盐和纯化.它具有简单、快速及纯度高的特点。
Molecular Sieve Chromatography in Fast Separating and Cleansing Cytochrome C 分子筛色谱法快速分离纯化细胞色素C
The expressed products exist mainly in the form of inclusion body and were purified by the molecular sieve and ion-exchange chromatography, then the recombinant protein was renatured by gradually decreasing the concentration of denaturant. 采用分子筛和离子交换层析对表达产物进行了纯化,获得了较高纯度的重组IL-1ra-Fcε融合蛋白,并采用逐步降低变性剂浓度的方式对融合蛋白进行了复性。
PAPP-A antigen in serum of pregnant women was purified by immunity chromatography, ion exchange chromatography and molecular sieve chromatography. 6. 抗原的纯化。足月孕妇血清依次用免疫亲和层析法,离子交换法以及分子筛法纯化。
Serum samples of full-term pregnant women were purified with affinity chromatography, ion exchange chromatography and molecular sieve chromatography. The PAPP-A was detected with SDS-PAGE, and a single band in the gel. 8. 足月孕妇血清经过亲和层析,离子交换以及分子筛层析之后,在SDS-PAGE检测的条带中呈现较为单一的条带,效果良好。8.检测不同孕期孕妇是否有DS。